Refereed publications from the Lee Laboratory.

Individuals working in the Lee Lab at the time work was done are indicated in bold.

 

PDFs for many of these publications can also be found under Robert Wingate Lee on ResearchGate <https://www.researchgate.net/profile/Robert_Lee2/contributions?ev=prf_act

 

 

2016

 

MacDonald, S. M. and Lee, R.W. (2016) A survey of Polytomella (Chlorophyceae, Chlorophyta) strains in public culture collections. J. Phycol. 52:656-663.


2015

 

MacDonald, S. M. and Lee, R.W. (2015) Validation of Polytomella piriformis nomen nudum (Chlamydomonadaceae): A distinct lineage within a genus of non-photosynthetic green algae. J. Eukaryot. Microbiol. 62:840-844.

View PDF.


2014

 

Smith, D.R., Lee, R.W. (2014) A plastid without a genome: evidence from the nonphotosynthetic green algal genus Polytomella. Plant Physiol. 164:1812-1819. View PDF.

 

Lee, R.W. and Hua, J. (2014) Mitochondrial Genomes of Green, Red and Glaucophyte Algae. In: Bell E., Bond J., Klinman J., Masters B., Wells R. (Ed.) Molecular Life Sciences: An Encyclopedic Reference: SpringerReference (www.springerreference.com). Springer-Verlag Berlin Heidelberg.  Reference no. 333645  232/232.

 

2013

 

Smith, D.R., Hua, J., Archibald, J.M., Lee, R.W. (2013) Palindromic genes in the linear mitochondrial genome of the nonphotosynthetic green alga Polytomella magna. Genome Biol Evol. 5:1661–1667. View PDF.

 

2012

 

Smith, D. R., Hua, J., Lee, R.W., Keeling, P.J. (2012) Relative rates of evolution among the three genetic compartments of the   red alga Porphyra differ from those of green plants and do not correlate with genome architecture. Molec. Phylogenet. Evol. 65:339-344. View PDF.

 

Hua, J. and Lee, R.W. (2012) Factors affecting codon bias in the mitochondrial genomes of the streptophyte Mesostigma viride and the chlorophyte Chlamydomonas reinhardtii. J. Euk. Microbiol. 59: 287-289. View PDF.

 

Hua, J., Smith, D.R., Borza T., and Lee R.W. (2012) Similar relative mutation rates in the three genetic compartments of Mesostigma and Chlamydomonas. Protist 163:105-115. View PDF.

 

2011

 

Smith, D.R. and Lee, R.W. (2011) Nucleotide diversity of the colorless green alga Polytomella parva (Chlorophyceae, Chlorophyta): high for the mitochondrial telomeres, surprisingly low everywhere else. J. Euk. Microbiol. 58:471-473. View PDF.

 

Smith D.R., Crosby, K., and Lee R.W. (2011) Correlation between nuclear plastid DNA abundance and plastid number supports the limited transfer window hypothesis. Genome Biol. Evol. 3: 365-371. (A commentary by Nick Lane Genome Biol Evol. 2011; 3:372-374 accompanies this article.)  View PDF.

 

2010

 

Smith, D.R., Hua, J. and Lee, R.W. (2010) Evolution of linear mitochondrial DNA in three known lineages of Polytomella. Curr. Genet. 56:427-438.  View PDF.

 

Smith, D.R. and Lee, R.W. (2010) Low nucleotide diversity for the expanded organelle and nuclear genomes of Volvox carteri supports the mutational-hazard hypothesis. Mol. Biol. Evol. 27:2244-2256 View PDF.

 

Smith, D.R., Lee, R.W., Cushman, J.C., Magnuson, J.K., Tran, D. and Polle, J.E.W. (2010) The Dunaliella salina organelle genomes: large sequences, inflated with intronic and intergenic DNA. BMC Plant Biol. 10:83.  View PDF.

 

2009

 

Smith, D.R. (2009) Unparalleled GC content in the plastid DNA of Selaginella. Plant Mol. Biol. 71:627-639.  View PDF.
This work was conducted in the laboratory of R.W. Lee and supported
by his NSERC grant.

 

Borza, T., Redmond, E.K., Laflamme, M. and Lee R.W. (2009) Mitochondrial DNA in the Oogamochlamys-clade (Chlorophyceae): high GC content and unique genome architecture for green algae. J. Phycol. 45:1323-1334 View PDF.

 

Smith, D.R. and Lee, R.W. (2009) Nucleotide diversity of the Chlamydomonas reinhardtii plastid genome: addressing the mutational-hazard hypothesis. BMC Evolutionary Biology 9:120 View PDF.

 

Smith, D.R. and Lee, R.W. (2009) The mitochondrial and plastid genomes of Volvox carteri: bloated molecules rich in repetitive DNA. BMC Genomics 10:132 View PDF.

 

2008

 

Smith, D.R. and Lee, R.W. (2008) Nucleotide diversity in the mitochondrial and nuclear compartments of Chlamydomonas reinhardtii: investigating the origins of genome architecture. BMC Evolutionary Biology 8:156.  View PDF.

 

Smith, D.R. and Lee, R.W. (2008) Mitochondrial genome of the colorless green alga Polytomella capuana: a linear molecule with an unprecedented GC content. Mol. Biol. Evol. 25:487-496.  View PDF.

 

2007

 

Koziol, A.G., Borza, T., Ishida, K.-I., Keeling, P., Lee, R.W. and D.G. Durnford (2007) Tracing the evolution of the light-harvesting antennae in chlorophyll a/b-containing organisms. Plant Physiol.143:1-15  View PDF.

 

Popescu, C.E and Lee, R.W.  (2007) Mitochondrial genome sequence evolution in Chlamydomonas. Genetics 175:819-826 
View PDF.

 

2006

 

Nedelcu, A.M.1, Borza, T.1, and Lee, R.W. (2006) A Plant-Specific Multigene Family in the Unicellar Mesostigma Argues for Its Close Relationship to Streptophyta. Mol. Biol. Evol. 23:1011-1015.  View PDF.  1These authors contributed equally to this work.

 

Popescu, C.E.,  Borza, T., Bielawski, J.P. and Lee, R.W. (2006) Evolutionary Rates and Expression Level in Chlamydomonas. Genetics 172:1567-1576  View PDF.

 

Mallet, M.A. and Lee, R.W. (2006) Identification of three distinct Polytomella lineages based on mitochondrial DNA features. J. Euk. Microbiol. 53: 79-84  View PDF.

 

2005

 

Keeling, P.J., Burger, G., Durnford, D.G., Lang, B.F., Lee, R.W., Pearlman, R.E., Roger, A. J. and Gray, M.W. (2005) The tree of eukaryotes. Trends Ecol. Evol. 20: 670-676  View PDF.

 

Borza, T., Popescu, C.E. and Lee, RW. (2005) Multiple metabolic roles for the nonphotosynthetic plastid of the green alga Prototheca wickerhamii. Euk. Cell 4:253-261  View PDF.

 

2003

 

Laflamme, M. and Lee, R.W. (2003) Mitochondrial genome conformation among CW-group chlorophycean algae. J. Phycol. 39, 213-220 (2003).  View PDF. 

 

Fan, J., Schnare, M.N. and Lee, R.W. (2003) Characterization of fragmented mitochondrial ribosomal RNAs of the colorless green alga Polytomella parva. Nucleic Acids Res. 31: 769-778  View PDF.

 

2002

 

Fan, J. and Lee, R.W. (2002) Mitochondrial genome of the colorless green alga Polytomella parva: Two linear DNA molecules with homologous inverted repeat termini. Mol. Biol. Evol. 19:999-1007  View PDF.

 

2000

 

Nedelcu, A.M. and Lee, R.W., Lemieux, C., Gray, M.W. and Burger, G. (2000) The complete mitochondrial DNA sequence of Scenedesmus obliquus reflects an intermediate stage in the evolution of the green algal mitochondrial genome. Genome Res. 10:819-831  View PDF.

 

1999 and earlier

 

Nedelcu, A.M. and Lee, R.W. (1998) Modes and tempos of mitochondrial and chloroplast genome evolution in Chlamydomonas: a comparative analysis. In: Rochaix, J.-D., M. Goldschmidt-Clermont and M. Merchant (Eds.), The Molecular Biology of Chlamydomonas: chloroplasts and mitochondria, pp. 63-91, Kluwer Academic Publishers, The Netherlands.

 

 Nedelcu, A.M. and Lee, R.W. (1998) A degenerate group II intron in the intronless mitochondrial genome of Chlamydomonas reinhardtii: Evolutionary implications. Mol. Biol. Evol. 15:918-922  

 

Nedelcu, A.M. and Lee, R.W. (1998) Short repetitive sequences in green algal mitochondrial genomes: Potential roles in mitochondrial genome evolution. Mol. Biol. Evol. 15:690-701 

 

Nedelcu, A,M (1998) Contrasting mitochondrial genome organizations and sequence affiliations among green algae: Potential factors, mechanisms, and evolutionary scenarios. J. Phycol. 34: 16-28 

 

Denovan-Wright, E.M., Nedelcu, A.M. and Lee, R.W. (1998) Complete sequence of the mitochondrial DNA of Chlamydomonas eugametos. Plant Mol. Biol. 36: 285-295

 

Nedelcu, A.M. (1997) Fragmented and scrambled mitochondrial ribosomal RNA coding regions among green algae: a model for their origin and evolution. Mol. Biol. Evol. 14:506-517 

 

Bussières, J., Lemieux, C., Lee, R.W., Turmel, M. (1996) Optional mobile genetic elements in the chloroplast DNAs of Chlamydomonas eugametos and C. moewusii: unidirectional gene conversions in high-viability  crosses. Curr. Genet. 30:356-365

 

Denovan-Wright, E.M., Sankoff, D. Spencer, D.F. and Lee, R.W. (1996) Evolution of fragmented ribosomal RNA genes in Chlamydomonas. J. Mol. Evol. 42:382-391

 

Nedelcu, A. M., Spencer, D.F., Denovan-Wright, E.M. and Lee, R.W. (1996) Discontinuous mitochondrial and chloroplast large subunit ribosomal RNAs among green algae: phylogenetic implications. J. Phycol. 32:103-111 

 

Denovan-Wright, E.M. and Lee, R.W. (1995) Evidence that the fragmented ribosomal RNAs of Chlamydomonas mitochondria are associated with ribosomes. FEBS Letters 370:222-226

 

Denovan-Wright, E..M. and Lee, R.W. (1994) Comparative structure and genomic organization of the discontinuous mitochondrial ribosomal RNA genes of Chlamydomonas eugametos and Chlamydomonas reinhardtii. J. Mol. Biol. 241:298-311 

 

Denovan-Wright, E.M. and Lee, R.W. (1993) Chlamydomonas eugametos mitochondrial genome. In Genetic Maps, 6th Edition, Book 2, Bacteria, Algae and Protozoa, S.J. O'Brien Ed., Cold Spring Harbor Press 

 

Denovan-Wright, E.M. and Lee, R.W. (1992) Comparative analysis of the mitochondrial genomes of Chlamydomonas eugametos and Chlamydomonas moewusii. Curr. Genet. 21:197-202 

 

Lee, R.W., Dumas, C. Lemieux, C. and Turmel, M. (1991) Cloning and characterization of the mitochondrial genome of Chlamydomonas moewusii.  Mol. Gen. Genetics 231:53-58 

 

Lee, R.W. and Lemieux, C. (1990) Loss of hybrid lethality during backcross programs involving Chlamydomonas eugametos and Chlamydomonas moewusii (Chlorophyceae). J. Phycol. 26, 376-380

 

Lee, R.W., B. Langille, C. Lemieux and Boer, P.H. (1990) Inheritance of mitochondrial and chloroplast genome markers in backcrosses of Chlamydomonas eugametos X Chlamydomonas moewusii hybrids. Curr. Genet. 17, 73-76  

 

Lemieux, C. and Lee, R.W. (1987) Non-reciprocal recombination between alleles of the chloroplast 23S rRNA gene in interspecific Chlamydomonas crosses. Proc. Natl. Acad. Sci. USA 84, 4166-4170

 

Lee, R.W. and Lemieux, C. (1986) Biparental inheritance of non-Mendelian gene markers in Chlamydomonas moewusii.  Genetics 113, 589-600   

 

Turmel, M., G. Bellemare, R.W. Lee and Lemieux, C. (1986)  A linear DNA molecule of 5.9 kilobase-pairs is highly homologous to the chloroplast DNA in the green alga Chlamydomonas moewusii. Plant Mol. Biol. 6, 313-319 

 

Vandermeulen, J.H. and Lee, R.W. (1986) Lack of mutagenic activity of crude and refined oil in the unicellular alga Chlamydomonas reinhardtii.  Bull. Env. Cont. Toxicol. 36, 250-253 

 

Lemieux, C., M. Turmel, R.W. Lee and Bellemare, G. (1985) A 21 kilobase-pair deletion/addition difference in the inverted repeat sequence of Chlamydomonas eugametos and C. moewusii chloroplast DNA.  Plant Mol. Biol. 5, 77-84          

 

Boer, P.H., L. Bonen, R.W. Lee and Gray, M.W. (1985)  Genes for respiratory chain proteins and ribosomal RNAs are present on a 16 kbp DNA species from Chlamydomonas reinhardtii mitochondria. Proc. Natl. Acad. Sci. USA 82, 3340-3344  

 

Lemieux, C., M. Turmel, V. Seligy and Lee, R.W. (l985) The large subunit of ribulose-1,5-bisphosphate carboxylase-oxygenase  is encoded in the inverted repeat sequence of the Chlamydomonas eugametos chloroplast genome.  Curr.Genet. 9, 139-145

 

Lemieux, C., M. Turmel, V. Seligy and Lee, R.W. (1984) A genetical approach to the physical mapping of chloroplast genes in Chlamydomonas. Can. J. Biochem.Cell Biol. 62, 225-229

 

Lemieux, C., M. Turmel, V. Seligy and Lee, R.W. (1984) Chloroplast DNA  recombination in interspecific hybrids of Chlamydomonas: Linkage between a nonMendelian locus for streptomycin resistance and restriction fragments coding for 16S rRNA. Proc. Natl. Acad. Sci. USA 81, 1164-1168

 

Lemieux, C., M. Turmel and Lee, R.W. (1981) Physical evidence for recombination of chloroplast DNA in hybrid progeny of Chlamydomonas eugametos and C. moewusii.  Curr. Genet. 3, 97-103   

 

Lemieux, C., M. Turmel and Lee, R.W. (1982) Physical evidence for recombination of chloroplast DNA in hybrid progeny of Chlamydomonas eugametos and   C. moewusii. Rosowski, J.R., Parker, B.C. (eds.), Selected Papers in Phycology II. Allen Press       

 

Turmel, M, C. Lemieux and Lee, R.W. (1981) Dispersive labelling of Chlamydomonas  chloroplast DNA in 15N-14N density transfer experiments. Curr. Genet. 4, 91-97 

 

Lemieux, C., M. Turmel and Lee, R.W. (1980) Characterization of chloroplast DNA in Chlamydomonas eugametos and C. moewusii and its inheritance in hybrid progeny.  Curr. Genet. 2, 139-147 

 

Turmel, M., C. Lemieux and R.W. Lee (1980) Net synthesis of chloroplast DNA throughout the synchronized cell-cycle of Chlamydomonas.  Curr. Genet. 2, 229-232 

 

Lee, R.W. and Haughn, G.W. (1980) Induction and segregation of chloroplast mutations in vegetative cell cultures of Chlamydomonas reinhardtii. Genetics 96,79-94 

 

Lee, R.W. and Sapp, J.A. (1978)  Nuclear mutation increases streptomycin and spectinomycin sensitivity in Chlamydomonas. Genetics 88, 643-650 (1978).

 

Whiteway, M.S. and Lee, R.W. (1977)  Chloroplast DNA content increases with nuclear ploidy in ChlamydomonasMol. Gen. Genetics 157, 11-15 

 

Lee, R.W. and Jones, R.F. (1976) Lethal and mutagenic effects of nitrosoguanidine on synchronized Chlamydomonas.  Mol. Gen. Genetics 147, 283-289 

 

Hawks, B.G. and Lee, R.W. (1976) Methyl methanesulfonate mutagenesis of synchronized ChlamydomonasMutation Res. 37, 222-228  

 

Gillham, N.W., J.E. Boynton and Lee, R.W. (1974) Segregation and recombination of non-Mendelian genes in ChlamydomonasGenetics 78, 439-457  

 

Lee, R.W. and Jones, R.F. (1973) Induction of Mendelian and non-Mendelian streptomycin resistant mutants during the synchronous cell cycle of Chlamydomonas reinhardtii. Mol. Gen. Genet.121, 99-108 

 

Lee, R.W., N.W. Gillham, K.P. Van Winkle and Boynton, J.E. (1973) Preferential recovery of uniparental streptomycin resistant mutants from diploid Chlamydomonas reinhardtii. Molec. Gen. Genetics 121, 109-116 

 

Yentsch, C.S. and Lee, R.W. (1966) A study of photosynthetic light reactions and a new interpretation of sun and shade phytoplankton. J. of Marine Res. 24, 319-337 

 

Wilce, R.T. and Lee, R.W. (1964) Lomentaria clavellosa in North America. Botanica Marina VI, 251-258